Enzyme immunoassay for quantitative determination of human TDP43 protein in biological samples.
The hTDP43 total ELISA is based on a sensitive sandwich ELISA using mAb 21B2 for capturing TDP43. The epitope of 21B2 is located in N-terminal region of TDP43 (amino acids 79-91). As detection antibody HRP labelled mAb 2G10 binding in the middle region of TDP43 is used. Amount of bound conjugated antibody is estimated using chromogenic substrate tetramethylbenzidine (TMB). The concentration of TDP43 is proportional to the obtained optical density. Controls are included for the proof of reproducibility and evaluation of the assay within labs.
Research use only